ABSTRACT
<p><b>OBJECTIVE</b>To be acquainted with genetic characteristics and variation of mumps virus strains circulating in Hunan province.</p><p><b>METHODS</b>Mumps virus (MV) strains were isolated using Vero/ SLAM cells. The small hydrophobic protein (SH) genes of MV isolates were sequenced, and the sequences were analysed phylogenetically between the isolated strains and other reference mumps strains.</p><p><b>RESULTS</b>4 mumps virus strains were isolated from 16 specimens collected in 2011 from different regions of Hunan province. The genotype of isolated strains were supposed to be F type.</p><p><b>CONCLUSION</b>Genotype F is the main genotype of circulating strains in Hunan province in 2011 and there is no variation between genotype.</p>
Subject(s)
Animals , Humans , Chlorocebus aethiops , China , Epidemiology , Genetic Variation , Genotype , Mumps , Epidemiology , Virology , Mumps virus , Genetics , Phylogeny , Vero Cells , Viral Proteins , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To study the global evolutionary characteristics of hemagglutinin gene HA1 of influenza H1N1 infecting different species during 2000-2009.</p><p><b>METHODS</b>The target sequences were downloaded from NCBI and analyzed using bioinformatic software to construct the phylogenetic tree.</p><p><b>RESULTS</b>The HA1 amino acid sequences of influenza H1N1 contained four mutated antigenic sites and receptor-binding sites, and the novel influenza virus shared most of the mutated amino acid sites with swine H1N1 influenza virus.</p><p><b>CONCLUSION</b>The HA1 gene of novel influenza virus might originate from the early swine H1N1 influenza virus from North America, and in the evolutionary process, a number of important sites of HA1 gene mutated to result in the outbreak of influenza.</p>